EcoClone T-Link
EcoClone T-Link is a compact, high-copy bacterial cloning and sequencing vector with kanamycin resistance. The 3,143 bp circular plasmid contains T7 and SP6 promoter regions flanking the cloning region, M13 forward and reverse sequencing primer sites, a lac promoter/operator region, a high-copy bacterial origin of replication, and an f1 origin. It is supplied as purified plasmid DNA for research use only.
Overview
- Catalog number
- PL0009
- Pack size
- 100 µL (5 µg total DNA)
- Shipping
- Ship at ambient temperature.
- Storage
- Store at -20°C.
EcoClone T-Link is a compact, high-copy bacterial cloning and sequencing vector with kanamycin resistance. The 3,143 bp circular plasmid contains T7 and SP6 promoter regions flanking the cloning region, M13 forward and reverse sequencing primer sites, a lac promoter/operator region, a high-copy bacterial origin of replication, and an f1 origin. It is supplied as purified plasmid DNA for research use only.
Reference backbone
pGEM-T
Vector Information
Vector size: 3143 bp; circular Promoter / regulatory elements: T7 promoter; SP6 promoter; lac promoter/operator Bacterial selection: Kanamycin [aph(3')-II / nptII] Mammalian selection: None Sequencing sites: M13 forward and reverse primer sites Origin of replication: High-copy ColE1/pMB1/pBR322/pUC-type ori; f1 ori
Applications
Routine DNA cloning and subcloning; Sequencing-template preparation using M13/T7/SP6 regions; In vitro transcription from T7 or SP6 promoters; Preparation of a TA-cloning backbone following appropriate linearization and 3′-T tailing; Plasmid propagation in compatible E. coli strains.
Multiple Cloning Site (MCS)
EcoRI - BamHI - ClaI - BamHI - HindIII
Important Note
For Research Use Only. Not for use in diagnostic procedures. The supplied material is circular plasmid DNA. For direct TA cloning of PCR products, the vector must first be linearized and prepared with compatible 3′-T overhangs. Restriction-site availability, insert orientation, and selection conditions should be verified for the intended cloning workflow.
