ClearPeak One Step qRT-PCR Probe Kit is a one-step Real-Time RT-qPCR kit designed for rapid and sensitive detection of RNA targets. 
 
Reverse transcription and quantitative PCR are performed consecutively within a single reaction mixture, eliminating the need for tube opening or additional reagent handling. This closed-tube format minimizes contamination risk and enhances workflow efficiency.
 
ClearPeak One Step qRT-PCR Probe Kit includes an advanced reverse transcriptase lacking RNase H activity, thereby preserving RNA integrity during cDNA synthesis. The enzyme exhibits high efficiency even with low RNA input and demonstrates strong performance with templates containing high GC content or complex secondary structures. 
 
To improve specificity, the reverse transcriptase remains inactive at lower temperatures and is activated during the initial stages of thermal cycling. This feature helps reduce non-specific amplification caused by primer–dimer formation or non-specific primer binding, leading to improved accuracy in quantitative analysis.
 
The optimized buffer system enables both reverse transcription and PCR amplification to occur efficiently within the same reaction, maximizing overall performance. The kit offers high sensitivity, excellent specificity, and a broad dynamic range for precise quantification of target genes.