EcoFluor EGFP-N
EcoFluor EGFP-N is a c-terminal egfp fusion vector designed for the molecular biology applications described below. It is supplied as purified plasmid DNA for research use only.
Overview
- Catalog number
- PL0003
- Pack size
- 100 µL (5 µg total DNA)
- Shipping
- Ship at ambient temperature.
- Storage
- Store at -20°C.
EcoFluor EGFP-N is a c-terminal egfp fusion vector designed for the molecular biology applications described below. It is supplied as purified plasmid DNA for research use only.
Reference backbone
pEGFP-N1
Vector Information
Vector size: 4733 bp Promoter: CMV promoter/enhancer Bacterial selection: Kanamycin Mammalian selection: Neomycin/G418 Reporter / tag: EGFP Origin of replication: bacterial ori; SV40 ori
Applications
Live-cell fluorescence imaging; Subcellular localization; Protein trafficking and dynamics; Fusion-protein expression studies.
Multiple Cloning Site (MCS)
BglII - XhoI - SacI - HindIII - EcoRI - PstI - SalI/AccI - Acc65I/KpnI - SacII - XmaI/ApaI/SmaI - BamHI - AgeI
Important Note
For Research Use Only. Not for use in diagnostic procedures. The MCS is upstream of EGFP. For a continuous protein-EGFP fusion, the insert should normally be cloned without its native stop codon and in the correct reading frame.
