EcoExpress T7-HisK
EcoExpress T7-HisK is a t7 his-tag bacterial expression vector, kanr designed for the molecular biology applications described below. It is supplied as purified plasmid DNA for research use only.
Overview
- Catalog number
- PL0004
- Pack size
- 100 µL (5 µg total DNA)
- Shipping
- Ship at ambient temperature.
- Storage
- Store at -20°C.
EcoExpress T7-HisK is a t7 his-tag bacterial expression vector, kanr designed for the molecular biology applications described below. It is supplied as purified plasmid DNA for research use only.
Reference backbone
pET-28a(+)
Vector Information
Vector size: 5369 bp Promoter: T7 promoter / lac operator Bacterial selection: Kanamycin Mammalian selection: None Reporter / tag: 6xHis; thrombin cleavage site Origin of replication: bacterial ori
Applications
Recombinant protein expression; Ni-NTA/IMAC purification; Enzyme production; Protein biochemical and functional studies.
Multiple Cloning Site (MCS)
XhoI - NotI - HindIII - SalI - SacI - EcoRI - BamHI - NheI - NdeI
Important Note
For Research Use Only. Not for use in diagnostic procedures. Use a compatible host supplying T7 RNA polymerase. The final His-tag arrangement depends on cloning sites, reading frame and presence or absence of an insert stop codon.
