ClearBand Bradford Reagent is formulated for a ready-to-use total protein analysis reagent used for quick measurement of total protein concentration. ClearBand Bradford Reagent contains Coomassie Brilliant Blue G-250, which associates with basic and aromatic amino acids, thus leading to a shift in absorbance during protein determination.ClearBand Bradford Reagent offer an easy-to-use assay in either test tube or microplate format: mix protein sample with the assay reagent, incubate shortly and measure the absorbance at 595nm. Color response with Coomassie is non-linear with increasing protein concentration, therefore, a standard curve must be created with each assay.