EcoSpin Plasmid Isolation Midi Kit is designed as a simple, convenient, and cost-effective purification of high-quality plasmid DNA from recombinant E. coli cultures. This kit utilizes chaotropic ions and silica-based membrane technology, eliminating the need for time-consuming alcohol precipitation method. The standard protocol lasts less than 60 minutes. The kit can be effectively used for purification of any size plasmids and cosmids. The relative plasmid yield and optimal culture size depend on the plasmid copy number and medium used for the bacterial culture.