ClearBand ECL Western Blotting Substrate is specifically formulated for highly sensitive, non-radioactive, enhanced luminol-based chemiluminescent substrate for easy detection of horseradish peroxidase (HRP) on immunoblots. ClearBand ECL Western Blotting Substrate offers excellent signal to noise ratio and clear background. ECL50 is sufficient for 400 cm²of membrane contents and ECL200 is sufficient for 1600 cm²of membrane contents.