Direct Blood EcoTaq DNA Polymerase is an engineered variant of Taq DNA polymerase, developed through N-terminal truncation and targeted mutations. This modification enhances tolerance to common PCR inhibitors, enabling direct DNA amplification from whole blood samples of human and mouse origin without the need for prior DNA extraction or purification.
 
Despite the relatively low DNA content and complexity of certain sample types, the enzyme is also suitable for direct DNA amplification from saliva, serum, and urine samples without the need for prior DNA extraction or purification. 
 
The enzyme generates PCR products with a 3′-A overhang, allowing direct use in T/A cloning applications. 
 
Direct Blood EcoTaq DNA Polymerase is supplied together with 10x Reaction Buffer. 10×PCR Buffer contains 30 mM MgCl2.