EcoTaq Ultra High-Fidelity DNA Polymerase is a next-generation, ultra high-fidelity DNA polymerase engineered from Pfu DNA Polymerase, offering significantly enhanced amplification efficiency, specificity, and yield. The enzyme is optimized for robust performance across a wide range of templates, including both simple and complex DNA sources.
 
With an optimized reaction buffer system, EcoTaq Ultra High-Fidelity DNA Polymerase enables efficient amplification of long DNA fragments. It can amplify fragments up to 40 kb from simple templates such as lambda DNA and plasmids, up to 20 kb from complex templates such as genomic DNA, and up to 10 kb from cDNA templates.
 
The enzyme exhibits an exceptionally low mismatch rate, approximately 1/53 that of standard Taq DNA polymerase and 1/6 that of conventional Pfu DNA polymerase, ensuring superior accuracy in DNA amplification. In addition, it features a rapid extension rate of approximately 15–30 seconds per kilobase, enabling faster PCR workflows without compromising fidelity.
 
EcoTaq Ultra High-Fidelity DNA Polymerase generates blunt-ended PCR products and is suitable for applications requiring high precision and reliability. It is particularly well-suited for demanding applications such as long-range PCR, cloning, sequencing, and mutagenesis etc.
 
EcoTaq DNA Polymerase is supplied together with 5x Reaction Buffer. 5x Reaction Buffer contains 10 mM MgCl2.