ClearPeak One Step qRT-PCR SYBR Kit is a one-step Real-Time RT-qPCR kit designed for rapid and sensitive detection of RNA targets. It utilizes SYBR Green I dye, which binds to double-stranded DNA, allowing detection of a wide range of target sequences without the need for sequence-specific probes. 
 
Reverse transcription and quantitative PCR are performed consecutively within a single reaction mixture, eliminating the need for tube opening or additional reagent handling. This closed-tube format minimizes contamination risk and enhances workflow efficiency.
 
ClearPeak One Step qRT-PCR SYBR Kit includes an advanced reverse transcriptase lacking RNase H activity, thereby preserving RNA integrity during cDNA synthesis. The enzyme exhibits high efficiency even with low RNA input and demonstrates strong performance with templates containing high GC content or complex secondary structures.